Identification of H3K4me1-associated proteins at mammalian enhancers.

Enhancers act to regulate cell-type-specific gene expression by facilitating the transcription of target genes. In mammalian cells, active or primed enhancers are commonly marked by monomethylation of histone H3 at lysine 4 (H3K4me1) in a cell-type-specific manner. Whether and how this histone modification regulates enhancer-dependent transcription programs in mammals is unclear. In this study, we conducted SILAC mass spectrometry experiments with mononucleosomes and identified multiple H3K4me1-associated proteins, including many involved in chromatin remodeling. We demonstrate that H3K4me1 augments association of the chromatin-remodeling complex BAF to enhancers in vivo and that, in vitro, H3K4me1-marked nucleosomes are more efficiently remodeled by the BAF complex. Crystal structures of the BAF component BAF45C indicate that monomethylation, but not trimethylation, is accommodated by BAF45C's H3K4-binding site. Our results suggest that H3K4me1 has an active role at enhancers by facilitating binding of the BAF complex and possibly other chromatin regulators

The large area detector onboard the eXTP mission

The Large Area Detector (LAD) is the high-throughput, spectral-timing instrument onboard the eXTP mission, a flagship mission of the Chinese Academy of Sciences and the China National Space Administration, with a large European participation coordinated by Italy and Spain. The eXTP mission is currently performing its phase B study, with a target launch at the end-2027. The eXTP scientific payload includes four instruments (SFA, PFA, LAD and WFM) offering unprecedented simultaneous wide-band X-ray timing and polarimetry sensitivity. The LAD instrument is based on the design originally proposed for the LOFT mission. It envisages a deployed 3.2 m2 effective area in the 2-30 keV energy range, achieved through the technology of the large-area Silicon Drift Detectors - offering a spectral resolution of up to 200 eV FWHM at 6 keV - and of capillary plate collimators - limiting the field of view to about 1 degree. In this paper we will provide an overview of the LAD instrument design, its current status of development and anticipated performance

Single-Stage Underwater Target Detection Based on Feature Anchor Frame Double Optimization Network

Objective: The shallow underwater environment is complex, with problems of color shift, uneven illumination, blurring, and distortion in the imaging process. These scenes are very unfavorable for the reasoning of the detection network. Additionally, typical object identification algorithms struggle to maintain high resilience in underwater environments due to picture domain offset, making underwater object detection problematic. Methods: This paper proposes a single-stage detection method with the double enhancement of anchor boxes and features. The feature context relevance is improved by proposing a composite-connected backbone network. The receptive field enhancement module is introduced to enhance the multi-scale detection capability. Finally, a prediction refinement strategy is proposed, which refines the anchor frame and features through two regressions, solves the problem of feature anchor frame misalignment, and improves the detection performance of the single-stage underwater algorithm. Results: We achieved an effect of 80.2 mAP on the Labeled Fish in the Wild dataset, which saves some computational resources and time while still improving accuracy. On the original basis, UWNet can achieve 2.1 AP accuracy improvement due to the powerful feature extraction function and the critical role of multi-scale functional modules. At an input resolution of 300 × 300, UWNet can provide an accuracy of 32.4 AP. When choosing the number of prediction layers, the accuracy of the four and six prediction layer structures is compared. The experiments show that on the Labeled Fish in the Wild dataset, the six prediction layers are better than the four. Conclusion: The single-stage underwater detection model UWNet proposed in this research has a double anchor frame and feature optimization. By adding three functional modules, the underwater detection of the single-stage detector is enhanced to address the issue that it is simple to miss detection while detecting small underwater targets

Classification of Infrared Objects in Manifold Space Using Kullback-Leibler Divergence of Gaussian Distributions of Image Points

Infrared image recognition technology can work day and night and has a long detection distance. However, the infrared objects have less prior information and external factors in the real-world environment easily interfere with them. Therefore, infrared object classification is a very challenging research area. Manifold learning can be used to improve the classification accuracy of infrared images in the manifold space. In this article, we propose a novel manifold learning algorithm for infrared object detection and classification. First, a manifold space is constructed with each pixel of the infrared object image as a dimension. Infrared images are represented as data points in this constructed manifold space. Next, we simulate the probability distribution information of infrared data points with the Gaussian distribution in the manifold space. Then, based on the Gaussian distribution information in the manifold space, the distribution characteristics of the data points of the infrared image in the low-dimensional space are derived. The proposed algorithm uses the Kullback-Leibler (KL) divergence to minimize the loss function between two symmetrical distributions, and finally completes the classification in the low-dimensional manifold space. The efficiency of the algorithm is validated on two public infrared image data sets. The experiments show that the proposed method has a 97.46% classification accuracy and competitive speed in regards to the analyzed data sets

A Robust Face Recognition Algorithm Based on an Improved Generative Confrontation Network

Objective: In practical applications, an image of a face is often partially occluded, which decreases the recognition rate and the robustness. Therefore, in response to this situation, an effective face recognition model based on an improved generative adversarial network (GAN) is proposed. Methods: First, we use a generator composed of an autoencoder and the adversarial learning of two discriminators (local discriminator and global discriminator) to fill and repair an occluded face image. On this basis, the Resnet-50 network is used to perform image restoration on the face. In our recognition framework, we introduce a classification loss function that can quantify the distance between classes. The image generated by the generator can only capture the rough shape of the missing facial components or generate the wrong pixels. To obtain a clearer and more realistic image, this paper uses two discriminators (local discriminator and global discriminator, as mentioned above). The images generated by the proposed method are coherent and minimally influence facial expression recognition. Through experiments, facial images with different occlusion conditions are compared before and after the facial expressions are filled, and the recognition rates of different algorithms are compared. Results: The images generated by the method in this paper are truly coherent and have little impact on facial expression recognition. When the occlusion area is less than 50%, the overall recognition rate of the model is above 80%, which is close to the recognition rate pertaining to the non-occluded images. Conclusions: The experimental results show that the method in this paper has a better restoration effect and higher recognition rate for face images of different occlusion types and regions. Furthermore, it can be used for face recognition in a daily occlusion environment, and achieve a better recognition effect

An approach for modelling software product line architecture

One of the challenges of the Software Product Line Architecture design is how to model and present the differences of the member products. Many approaches have been introduced, such as FORM, FODA, KobrA etc. In this paper, we propose an approach to transform feature models into architecture models. This iterative approach explicitly models the variability presented in the feature model into architectural artifacts and transfers the feature dependencies into the interactions between the architectural artifacts in the architecture model. The approach improves the traceability between the feature models and architecture models, thus provide better guidance for the architecture development of the member product in Software Product Lines